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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TPO (Thrombopoietin) protein- GMP grade

EC50

100-500 ng/mL EC50 in MO7e cells in defined media

Specific Activity

>1.00 x 106 IU/mg

Species Reactivity

human

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1248-GMP

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Validation Data Gallery

  • Purity of GMP-grade recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP-grade recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human TPO (HZ-1248-GMP) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of GMP recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 100-500 ng/mL​.

    GMP Recombinant human TPO (HZ-1248-GMP) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of GMP recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 100-500 ng/mL​.

Technical Specifications

GeneID 7066
Species Human
Expression HEK293
EC50 100-500 ng/mL EC50 in MO7e cells in defined media
Specific Activity>1.00 x 106 IU/mg
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP40225
Molecular Mass 70 to 93 kDa reduced and non-reduced, monomer, glycosylated.
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing pH 7.4 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

TPO (Thrombopoietin) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin