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Animal-free Activin A Human Recombinant23 文献

カタログ番号: HZ-1138

HEK293 由来 アニマルフリー エンドトキシンフリー

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（希望販売価格）

形状: 凍結乾燥品

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製品情報

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The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

GeneID:
3624
動物種:
Human
発現細胞:
HEK293
活性:
0.5-3.5 ng/mL EC50
純度:
>95%
分子量:
25 kDa, homodimer, non-glycosylated
組成
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human,mouse

再溶解と保存方法

安定性と保存方法:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


再溶解:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

製品詳細

詳細:

Animal-free Recombinant Human Activin A is a member of the TGF beta superfamily, expressed from human 293 cells as disulfide linked homodimer with an apparent molecular mass of 25 kDa. This cytokine is produced in a serum-free, chemically defined media.



背景説明:

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.



別名:
Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A



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