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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BMP-7 protein

Activity

50-275 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1229

Synonyms

BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, HZ1229, OP 1, OP1, Osteogenic protein 1

Validation Data Gallery

  • Purity of recombinant human BMP-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human BMP-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human BMP-7 (HZ-1229) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-7 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 3.6-18.5 ng/ml.

    Recombinant human BMP-7 (HZ-1229) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-7 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 3.6-18.5 ng/ml.

Technical Specifications

GeneID 655
Species Human
Expression HEK293
Activity 50-275 ng/mL
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP18075
Molecular Mass 20 kDa reduced, 32 to 36 kDa non-reduced, homodimer, glycosylated
Formulation 10mM Acetic Acid
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in 10mM Acetic Acid containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Bone morphogenetic protein-7 (BMP7), also known as osteogenic protein-1 or OP-1, plays a key role in the differentiation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in several cancers, including breast, prostate, and colon cancers (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients is significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

Synonyms

BMP 7, BMP7, BMP-7, bone morphogenetic protein 7, Eptotermin alfa, HZ1229, OP 1, OP1, Osteogenic protein 1

Publications

SpeciesTitle
human

Stem Cell Res Ther

Generation of special autosomal dominant polycystic kidney disease iPSCs with the capability of functional kidney-like cell differentiation.

Authors - JiahuiHuang
View Article

J Tissue Eng Regen Med

An Easy Long-acting BMP7 Release System Based on Biopolymer Nanoparticles for Inducing Osteogenic Differentiation of Adipose Mesenchymal Stem Cells.

Authors - Rui Chen
View Article
human

Biochem Biophys Res Commun

Angiopoietin-like 4 enhances metastasis and inhibits apoptosis via inducing bone morphogenetic protein 7 in colorectal cancer cells.

Authors - XuquanLi
View Article

Neuroscience

BMP5 expression in the adult rat brain.

Authors - YKusakawa
View Article
human

Biomed Mater

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity.

Authors - Xiao-Hong Zhao
View Article
human

Regen Med

Seven diverse human embryonic stem cell-derived chondrogenic clonal embryonic progenitor cell lines display site-specific cell fates.

Authors - HalSternberg
View Article