• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-4 protein

Activity

≤ 1.25 ng/mL in Balb/c 3T3 cells. 6-30 ng/mL in NIH 3T3 cells in defined media

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1218

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3



Technical Specifications

GeneID 2249
Species Human
Expression HEK293
Activity ≤ 1.25 ng/mL in Balb/c 3T3 cells. 6-30 ng/mL in NIH 3T3 cells in defined media
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP11403
Molecular Mass 17 and 27 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

Background

Fibroblast growth factor-4 (FGF-4) plays an important physiologically role in the self-renewal of human embryonic stem cells (PMID 21990129). It also promotes stem cell proliferation and may aid in differentiation. Xeno-free Recombinant Human FGF-4 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 17 and 27 kDa.

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

Publications

SpeciesTitle
mouse

J Cell Commun Signal

Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.

Authors - Yin Cao