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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-8b protein

Activity

10-60 ng/mL EC50 in NIH 3T3 in defined media or 0.7-4 ng/mL EC50 in Balb/c 3T3.

Species Reactivity

human,mouse,pig

Purity

>95%

Cat no : HZ-1103

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, FGF-8b, Fibroblast growth factor 8, HBGF 8, KAL6

Validation Data Gallery

  • Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

  • Recombinant human FGF-8b (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.

    Recombinant human FGF-8b (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.

  • Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

    Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

Technical Specifications

GeneID 2253
Species Human
Expression HEK293
Activity 10-60 ng/mL EC50 in NIH 3T3 in defined media or 0.7-4 ng/mL EC50 in Balb/c 3T3.
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP55075
Molecular Mass 30 to 45 kDa reduced and non-reduced, monomer, glycosylated
Formulation 10 mM Tris-HCl pH 7.4 + 800 mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse,pig

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Fibroblast growth factor 8 (FGF8), also known as AIGF and HBGF-8, belongs to the FGF family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (PMID: 11276432; 15863033). FGF8 stimulates growth in an autocrine manner in normal individuals but mediates hormonal action during tumorigenesis (PMID 17512240). Defects in FGF8 cause Kallmann syndrome type 6 (KAL6) and idiopathic hypogonadotropic hypogonadism (IHH) (PMID 18596921).

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, FGF-8b, Fibroblast growth factor 8, HBGF 8, KAL6

Publications

SpeciesTitle
human

Stem Cells Transl Med

Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo.

Authors - Benjamin M Hiller
View Article
pig

NMR Biomed

MRI study of cryoinjury infarction in pig hearts: i. Effects of intrapericardial delivery of bFGF/VEGF embedded in alginate beads.

Authors - YYang
View Article