• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FLT3 Ligand protein- GMP grade

EC50

0.4-3.0 ng/mL

Specific Activity

minimally 7.00 x 105 IU/mg, typically 7.24 x 105 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1151-GMP

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine



Technical Specifications

GeneID 2323
Species Human
Expression HEK293
EC50 0.4-3.0 ng/mL
Specific Activityminimally 7.00 x 105 IU/mg, typically 7.24 x 105 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP49771
Molecular Mass 27 to 34 kDa reduced, 23 to 30 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID: 8590775; 7544638; 12176867). GMP grade Humankine® Recombinant Human FLT3 ligand was expressed and purified tag free from HEK293 human cell line without the use of any animal components.

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine