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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human HGF protein

Activity

5-25 ng/mL

Specific Activity

minimally 2.50 x 105 IU/mg

Species Reactivity

human,monkey,mouse,canine,rat,zebrafish

Purity

>95%

Cat no : HZ-1084

Synonyms

F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF

Validation Data Gallery

  • Purity of recombinant human HGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human HGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • The activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 3082
Species Human
Expression HEK293
Activity 5-25 ng/mL
Specific Activityminimally 2.50 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP14210
Molecular Mass 85 kDa reduced, 70 kDa non-reduced, single chain, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,monkey,mouse,canine,rat,zebrafish

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. It is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin (PMID 1530787, 2531289). HGF exerts a variety of effects on several cells, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities (PMID: 1846706; 15070743; 1383237; 1827664).

Synonyms

F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF

Publications

SpeciesTitle
canine

J Cell Biol

Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells.

Authors - ChristophKlingner
View Article
human

Pharmacol Res

The natural product berberine synergizes with osimertinib preferentially against MET-amplified osimertinib-resistant lung cancer via direct MET inhibition

Authors - Zhen Chen
View Article
human

Toxicol Sci

In Silico Modeling for the Prediction of Dose and Pathway-Related Adverse Effects in Humans From In Vitro Repeated-Dose Studies.

Authors - SebastianKlein
View Article
zebrafish

Toxicol Lett

Tools for studying the metabolism of new psychoactive substances for toxicological screening purposes - A comparative study using pooled human liver S9, HepaRG cells, and zebrafish larvae.

Authors - Lilian H J Richter
View Article
human

Anal Bioanal Chem

Metabolic fate of desomorphine elucidated using rat urine, pooled human liver preparations, and human hepatocyte cultures as well as its detectability using standard urine screening approaches.

Authors - Lilian H JRichter
View Article
human

J Pharm Biomed Anal

Pooled human liver preparations, HepaRG, or HepG2 cell lines for metabolism studies of new psychoactive substances? A study using MDMA, MDBD, butylone, MDPPP, MDPV, MDPB, 5-MAPB, and 5-API as examples.

Authors - Lilian H JRichter
View Article