- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IL-23 protein
Activity
0.2-1.2 ng/mL in HEK293 reporter cell line, 1.25-6.5 ng/mL in murine splenocytes
Species Reactivity
human,mouse
Purity
>95%
Cat no : HZ-1254
Synonyms
IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF
Validation Data Gallery
Technical Specifications
| GeneID | 51561,3593 |
| Species | Human |
| Expression | HEK293 |
| Activity | 0.2-1.2 ng/mL in HEK293 reporter cell line, 1.25-6.5 ng/mL in murine splenocytes |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | Q9NPF7 |
| Molecular Mass | 20 and 42 kDa reduced, 53 kDa non-reduced, heterodimer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially are stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages (PMID: 16393998;15657292;12023369;12610626)
Synonyms
IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF
Publications
| Species | Title |
|---|---|
Ann Rheum Dis JAK selectivity and the implications for clinical inhibition of pharmacodynamic cytokine signalling by filgotinib, upadacitinib, tofacitinib and baricitinib. | |
Cell Rep Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1. | |
Br J Pharmacol The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammation | |
J Mol Cell Cardiol Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis. | |
J Immunol The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species. | |
Sci Rep Functional defects in CD4+ CD25high FoxP3+ regulatory cells in ankylosing spondylitis. |