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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-27 protein

Activity

15-200 ng/mL in OCI-AML5 cells

Species Reactivity

human

Purity

>95%

Cat no : HZ-1275

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

Validation Data Gallery

  • Purity of recombinant human IL-27 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-27 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-27 (HZ-1275) stimulates dose-dependent proliferation of the OCI-AML5 (human acute myeloid leukemia) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human IL-27 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 15-200 ng/mL.

    Recombinant human IL-27 (HZ-1275) stimulates dose-dependent proliferation of the OCI-AML5 (human acute myeloid leukemia) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human IL-27 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 15-200 ng/mL.

Technical Specifications

GeneID 246778
Species Human
Expression HEK293
Activity 15-200 ng/mL in OCI-AML5 cells
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ8NEV9
Molecular Mass 25 and 30 kDa reduced and non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases (PMID: 22608254;16081786;16670296;16906167)

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha, p28

Publications

SpeciesTitle
human

J Exp Med

IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation.

Authors - LueDai
View Article
human

Stem Cell Res Ther

hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.

Authors - Aiping Zhang
View Article