- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IL-4 protein
Activity
0.07-0.4 ng/mL
Specific Activity
minimally 2 x 106 IU/mg
Species Reactivity
human,mouse
Purity
>95%
Cat no : HZ-1004
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra
Validation Data Gallery
Technical Specifications
| GeneID | 3565 |
| Species | Human |
| Expression | HEK293 |
| Activity | 0.07-0.4 ng/mL |
| Specific Activity | minimally 2 x 106 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P05112 |
| Molecular Mass | 15 and 21 kDa reduced, 13 and 19 kDa non-reduced, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival (PMID: 24489573;3049907;21663408)
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra
Publications
| Species | Title |
|---|---|
Cancer Commun (Lond) CD24 is a promising immunotherapeutic target for enhancing efficacy of third-generation EGFR-TKIs on EGFR-mutated lung cancer. | |
Bioact Mater Chronic inflammation-responsive hydrogel restores myeloid-T cell crosstalk to reinvigorate antitumor immunity against metastatic colorectal cancer. | |
Drug Resist Updat Targeting keratin 6 A overcomes gemcitabine resistance by restoring equilibrative nucleoside transporter 1 and TAM-mediated metabolic compensation in pancreatic cancer. | |
Nat Commun Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection | |
Nat Commun Time-staggered chemo-immunotherapy via engineered nanofiber resists postoperative dynamic immunosuppression in glioblastoma. | |
ACS Nano A Dual-Fusogenic Virus-like Vector Enables Direct Cytosolic Delivery of mRNA Vaccines to Dendritic Cells. |