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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-6 protein

Activity

0.03-0.24 ng/mL in 3G12B10 cells.

Specific Activity

minimally 3.00 x 107 IU/mg

Species Reactivity

human,mouse,monkey,rabbit,rat

Purity

>95%

Cat no : HZ-1019

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Validation Data Gallery

  • Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL​.

    Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL​.

  • Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL​.

    Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL​.

Technical Specifications

GeneID 3569
Species Human
Expression HEK293
Activity 0.03-0.24 ng/mL in 3G12B10 cells.
Specific Activityminimally 3.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP05231
Molecular Mass 24 and 29 kDa reduced, 21 and 28 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,monkey,rabbit,rat

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages. IL-6 plays an essential role in the final differentiation of B-cells into immunoglobulin-secreting cells. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity (PMID: 29736207; 29731693).

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Publications

SpeciesTitle
mouse

Small Methods

Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy

Authors - Shilin Li
View Article
human

J Exp Med

Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.

Authors - AndrewVolk
View Article
human

Cell Rep

Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1.

Authors - YusukeEndo
View Article
human

Mol Metab

IL-6/JAK2-dependent G6PD phosphorylation promotes nucleotide synthesis and supports tumor growth

Authors - Xuemei Qiu
View Article
human

Front Immunol

Dendritic cell phenotype and function in a 3D co-culture model of patient-derived metastatic colorectal cancer organoids

Authors - Beatriz Subtil
View Article
human

Cancers (Basel)

Silibinin Suppresses Tumor Cell-Intrinsic Resistance to Nintedanib and Enhances Its Clinical Activity in Lung Cancer.

Authors - Joaquim Bosch-Barrera
View Article