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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human LEFTY-1 protein

Activity

Typically ≤ 2.5 µg/mL

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1109

Synonyms

LEFTB, LEFTY1, LEFTYB, Protein lefty 1, Protein lefty B

Validation Data Gallery

  • Purity of recombinant human Lefty-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human Lefty-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • The activity of Lefty-1 was determined by the dose-dependent inhibition of proliferation of the Mv1Lu cell line (mink lung epithelial cells) using ProMega CellTiter96® aqueous Non-radioactive cell proliferation assay.

    The activity of Lefty-1 was determined by the dose-dependent inhibition of proliferation of the Mv1Lu cell line (mink lung epithelial cells) using ProMega CellTiter96® aqueous Non-radioactive cell proliferation assay.

Technical Specifications

GeneID 10637
Species Human
Expression HEK293
Activity Typically ≤ 2.5 µg/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberO75610
Molecular Mass 43 kDa reduced, 40 and 43 kDa non-reduced, monomer, glycosylated
Formulation 10 mM Tris pH 7.4 + 450 mM NaCl + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

LEFTY-1 is a member of the TGF-beta family of proteins that plays a role in left-right asymmetry determination of organ systems during development. LEFTY-1 regulates transforming growth factor, mitogen-activated protein kinase and other signaling pathways, and is considered to have a potential anti-inflammatory function (PMID: 29286065; 26316705; 10500184; 9708731)

Synonyms

LEFTB, LEFTY1, LEFTYB, Protein lefty 1, Protein lefty B

Publications

SpeciesTitle
human

Stem Cells Dev

Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.

Authors - ZhilaKhalkhali-Ellis
View Article