• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human LIF protein

Activity

0.135-0.675 ng/mL TF-1 Cell assay. 0.045-0.25 ng/mL EC50 in M1 cell assay

Specific Activity

minimally 3.50 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1292

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI



Technical Specifications

GeneID 3976
Species Human
Expression HEK293
Activity 0.135-0.675 ng/mL TF-1 Cell assay. 0.045-0.25 ng/mL EC50 in M1 cell assay
Specific Activityminimally 3.50 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP15018
Molecular Mass 35-55 kDa reduced and non-reduced, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

Publications

SpeciesTitle
mouse

Nucleic Acids Res

Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs.

Authors - Mahmoud-Reza Rafiee

Reprod Biol Endocrinol

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium.

Authors - Yue Liu

Brain Commun

Human stem cell-derived ventral midbrain astrocytes exhibit a region-specific secretory profile

Authors - Lucy A Crompton
human

Biochem Pharmacol

Enhanced binding of β-catenin and β-TrCP mediates LMPt's anti-CSCs activity in colorectal cancer

Authors - Mengyan Wang