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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human M-CSF protein

Activity

0.7-4.0 ng/mL

Specific Activity

minimally 4.00 x 107 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1192

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Validation Data Gallery

  • Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human M-CSF (Cat no: HZ-1192) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

    Recombinant human M-CSF (Cat no: HZ-1192) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

  • Proteintech M-CSF (Cat no: HZ-1192) demonstrates equivalent fold induction and a 2-fold better EC50 compared to leading competitors. Recombinant human M-CSF stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

    Proteintech M-CSF (Cat no: HZ-1192) demonstrates equivalent fold induction and a 2-fold better EC50 compared to leading competitors. Recombinant human M-CSF stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

Technical Specifications

GeneID 1435
Species Human
Expression HEK293
Activity 0.7-4.0 ng/mL
Specific Activityminimally 4.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP09603
Molecular Mass 19 and 23 kDa reduced, 35 and 40 kDa non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Publications

SpeciesTitle
human

Cell Metab

Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK

Authors - Yajuan Li
View Article
mouse

Cell Metab

Senescent immune cells release grancalcin to promote skeletal aging.

Authors - Chang-Jun Li
View Article
mouse

Cell Metab

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Authors - Hui Peng
View Article
mouse

ACS Nano

Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis.

Authors - Yongzhi Cui
View Article
mouse

Adv Sci (Weinh)

Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6

Authors - Chao Wei
View Article
human

Autophagy

Induction of a Na+/K+-ATPase-dependent form of autophagy triggers preferential cell death of human immunodeficiency virus type-1-infected macrophages.

Authors - Gang Zhang
View Article