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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human OSM protein

Activity

0.1-1.5 ng/mL

Specific Activity

minimally 3.00 x 107 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1030

Synonyms

oncostatin M, OSM

Validation Data Gallery

  • Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human OSM (HZ-1030) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.5 ng/mL​.

    Recombinant human OSM (HZ-1030) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.5 ng/mL​.

Technical Specifications

GeneID 5008
Species Human
Expression HEK293
Activity 0.1-1.5 ng/mL
Specific Activityminimally 3.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP13725
Molecular Mass 32 kDa reduced, 28 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Oncostatin M (OSM) is a member of a cytokine family that includes a leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. OSM is a growth regulator which inhibits the proliferation of some tumor cell lines. It can stimulate proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF, and GM-CSF from endothelial cells. OSM is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (PMID: 9133674; 29526757; 29363180; 21887386).

Synonyms

oncostatin M, OSM

Publications

SpeciesTitle
human

Oncotarget

CM363, a novel naphthoquinone derivative which acts as multikinase modulator and overcomes imatinib resistance in chronic myelogenous leukemia.

Authors - BorjaGuerra
View Article
human

Invest Ophthalmol Vis Sci

Role of Oncostatin M in the Pathogenesis of Vernal Keratoconjunctivitis: Focus on the Barrier Function of the Epithelium and Interleukin-33 Production by Fibroblasts

Authors - Ishin Ninomiya
View Article
human

Cancer Biomark

Interleukin-6 and oncostatin M are elevated in liver disease in conjunction with candidate hepatocellular carcinoma biomarker GP73.

Authors - HongyanLiang
View Article
human

Int J Dermatol

Livin upregulation in keratinocytes of psoriasis patients to promote adhesion molecule expression

Authors - Delu Che
View Article
human

Skin Res Technol

Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis

Authors - Delu Che
View Article
human

Front Pharmacol

A Novel Naphthoquinone-Coumarin Hybrid That Inhibits BCR-ABL1-STAT5 Oncogenic Pathway and Reduces Survival in Imatinib-Resistant Chronic Myelogenous Leukemia Cells.

Authors - Patricia Martín-Rodríguez
View Article