- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human PDGFaa protein
Activity
Typically ≤ 10 ng/mL in 3T3 cell, 150 - 900 ng/mL in NIH 3T3
Species Reactivity
human,mouse,rat
Purity
>95%
Cat no : HZ-1215
Synonyms
PDGF 1, PDGF A, PDGF subunit A, PDGF1, PDGFA, PDGFaa
Validation Data Gallery
Technical Specifications
| GeneID | 5154 |
| Species | Human |
| Expression | HEK293 |
| Activity | Typically ≤ 10 ng/mL in 3T3 cell, 150 - 900 ng/mL in NIH 3T3 |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P04085 |
| Molecular Mass | 19 and 26 reduced, 35, 40 and 45 kDa non-reduced, homodimer, glycosylated |
| Formulation | 10 mM HOAc, See Certificate of Analysis for details |
| Species Reactivity | human,mouse,rat |
Stability and Reconstitution
| Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 10 mM HOAc containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
PDGFaa is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFaa plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for a normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFaa is also involved in oligodendrocyte development and l myelination process in the spinal cord and cerebellum PMID: 29408302; 29282077; 27002148).
Synonyms
PDGF 1, PDGF A, PDGF subunit A, PDGF1, PDGFA, PDGFaa
Publications
| Species | Title |
|---|---|
Phytomedicine Kaempferol attenuates carbon tetrachloride (CCl4)-induced hepatic fibrosis by promoting ASIC1a degradation and suppression of the ASIC1a-mediated ERS | |
Oxid Med Cell Longev Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress. | |
Stem Cell Reports Conversion of Fibroblast into Functional Leydig-like Cell Using Defined Small Molecules. | |
Front Cell Neurosci Nicotinamide enhances myelin production after demyelination through reduction of astrogliosis and microgliosis | |
Nat Biotechnol Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain | |
Biol Chem Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy |