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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TGF beta 1 protein

Activity

Typically ≤ 0.5 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1011

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 7040
Species Human
Expression HEK293
Activity Typically ≤ 0.5 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP01137
Molecular Mass 25 kDa, homodimer, nonglycosylated
Formulation 50 mM NaOAc pH 4.5, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, and cell differentiation. TGF acts synergistically with TGFA in inducing transformation process. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB having specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling being a potent stimulator of an osteoblastic bone formation (PMID: 11586292).

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Publications

SpeciesTitle
human

J Biol Chem

Transforming growth factor β (TGF-β)-induced up-regulation of TGF-β receptors at the cell surface amplifies the TGF-β response.

Authors - Dana Duan
View Article
human

PLoS Biol

Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.

Authors - Lina Fu
View Article
human

Protein Cell

Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.

Authors - Xing Zhang
View Article

Immunity

A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells.

Authors - Frédéric Van Gool
View Article
human

J Cell Biochem

microRNA-539 functions as a tumor suppressor in papillary thyroid carcinoma via the transforming growth factor β1/Smads signaling pathway by targeting secretory leukocyte protease inhibitor.

Authors - Cheng-Bi Xu
View Article
human

Cell Stem Cell

FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration.

Authors - Pengze Yan
View Article