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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TPO (Thrombopoietin) protein

Activity

100-500 ng/mL EC50 in MO7e cells in defined media

Specific Activity

>1.00 x 106 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1248

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Validation Data Gallery

  • Purity of recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

  • Recombinant human TPO (HZ-1248) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of GMP recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL​.

    Recombinant human TPO (HZ-1248) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of GMP recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL​.

  • Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

    Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

Technical Specifications

GeneID 7066
Species Human
Expression HEK293
Activity 100-500 ng/mL EC50 in MO7e cells in defined media
Specific Activity>1.00 x 106 IU/mg
Purity >95%
Endotoxin< 1 EU/µg
Accession NumberP40225
Molecular Mass 70 to 93 kDa reduced and non-reduced, monomer, glycosylated.
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

TPO (Thrombopoietin) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Publications

SpeciesTitle
human

Blood

PF4 activates the c-Mpl-Jak2 pathway in platelets

Authors - Richard John Buka
View Article
human

J Exp Med

Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.

Authors - AndrewVolk
View Article
human

J Cell Physiol

HCMV modulates c-Mpl/IEX-1 pathway-mediated megakaryo/thrombopoiesis via PDGFRα and αvβ3 receptors after allo-HSCT.

Authors - Fei-Er Feng
View Article
human

ACS Biomater Sci Eng

Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.

Authors - Lidan Chen
View Article
human

Oncotarget

Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.

Authors - JingLi
View Article
human

Sci Rep

A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.

Authors - Dezhi Zhou
View Article