Validation Data Gallery

Filter: AllWBIHCIF-PIFFC (Intra)

WB analysis using 80008-1-RR (same clone as 80008-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis using 80008-1-RR (same clone as 80008-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 80008-1-RR (same clone as 80008-1-PBS)
WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis of zebrafish using 80008-1-RR (same clone as 80008-1-PBS)
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
WB analysis using 80008-1-RR (same clone as 80008-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human rectal cancer using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 80008-1-RR (same clone as 80008-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
IF Staining of mouse embryo using 80008-1-RR (same clone as 80008-1-PBS)
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
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E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
FC experiment of C2C12 using 80008-1-RR (same clone as 80008-1-PBS)
1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.
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1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Alpha smooth muscle actin specific Recombinant antibody, PBS Only

Alpha smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Cat No. 80008-1-PBS

Clone No.5H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80008-1-PBS targets Alpha smooth muscle actin specific in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

Tested Reactivity	human, mouse, rat, pig, chicken, zebrafish
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated molecular weight	42 kDa
Observed molecular weight	42 kDa
GenBank accession number	NM_001613
Gene Symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2882938
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P62736
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 80008-1-RR (same clone as 80008-1-PBS)

WB analysis using 80008-1-RR (same clone as 80008-1-PBS)

WB analysis of A549 using 80008-1-RR (same clone as 80008-1-PBS)

WB analysis of zebrafish using 80008-1-RR (same clone as 80008-1-PBS)

WB analysis using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human rectal cancer using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of mouse colon using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of rat colon using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)

IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)

IF Staining of mouse heart using 80008-1-RR (same clone as 80008-1-PBS)

IF Staining of mouse embryo using 80008-1-RR (same clone as 80008-1-PBS)

FC experiment of C2C12 using 80008-1-RR (same clone as 80008-1-PBS)

Alpha smooth muscle actin specific Recombinant antibody, PBS Only

Alpha smooth muscle actin specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Alpha smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA