SARS-CoV-2 S protein (428-506 aa) Polyclonal antibody
SARS-CoV-2 S protein (428-506 aa) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
virus
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28901-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive ELISA detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28901-1-AP targets SARS-CoV-2 S protein (428-506 aa) in WB, ELISA applications and shows reactivity with virus samples.
| Tested Reactivity | virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SARS-CoV-2 S protein (428-506 aa) fusion protein Ag30684 相同性解析による交差性が予測される生物種 |
| Full Name | SARS-CoV-2 Spike Protein |
| Calculated molecular weight | 141 kDa |
| GenBank accession number | NC_045512 |
| Gene symbol | SARS-COV-2 |
| Gene ID (NCBI) | 43740568 |
| RRID | AB_2881231 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 S protein (428-506 aa) antibody 28901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Irene (Verified Customer) (10-16-2020) | Tested the antibody for IF and western blot and it did not work for either application. There were minimal colocalization but also non-specific binding for IF.
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