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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Virus spike protein Recombinant antibody, PBS Only

spike protein Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

4I18

Cat no : 82734-4-PBS

Synonyms

RBD, COVID-19 RBD, Spike, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Recombinant protein using spike protein Recombinant antibody (82734-4-RR)

    WB analysis of Recombinant protein using 82734-4-RR (same clone as 82734-4-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 82734-4-RR (spike protein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82734-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82734-4-RR

    Affinity and Kinetic Characterization of 82734-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82734-4-RR against Virus spike protein were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82734-4-PBS targets spike protein in Indirect ELISA, WB applications and shows reactivity with virus samples.

Tested Reactivity virus
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen spike protein fusion protein Ag30684 相同性解析による交差性が予測される生物種
Full Name SARS-CoV-2 Spike Protein
Calculated molecular weight 141 kDa
GenBank accession numberNC_045512
Gene symbol SARS-COV-2
Gene ID (NCBI) 43740568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.