• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig stomach tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis of pig stomach using 60213-1-Ig (same clone as 60213-1-PBS)

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis of rat colon using 60213-1-Ig (same clone as 60213-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat stomach tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis of rat stomach using 60213-1-Ig (same clone as 60213-1-PBS)

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    WB analysis of human stomach using 60213-1-Ig (same clone as 60213-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using transgelin/SM22 Monoclonal antibody (60213-1-Ig)

    IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

transgelin/SM22 Monoclonal antibody, PBS Only

transgelin/SM22 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60213-1-PBS
Clone No.2A10C2

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, transgelin/SM22 alpha, WS3 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60213-1-PBS targets transgelin/SM22 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

Tested Reactivity human, rat, pig, mouse, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 18+22 kDa
GenBank accession numberBC004927
Gene Symbol transgelin/SM22
Gene ID (NCBI) 6876
RRIDAB_11043177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01995
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.