transgelin/SM22 Monoclonal antibody, PBS Only

Name: transgelin/SM22 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 60213-1-PBS

transgelin/SM22 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C2

Review Product

Cat no : 60213-1-PBS

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, transgelin/SM22 alpha, WS3 10

Validation Data Gallery

Filter:

AllWBIHCIF

WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis of human stomach using 60213-1-Ig (same clone as 60213-1-PBS)
human stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis of pig stomach using 60213-1-Ig (same clone as 60213-1-PBS)
pig stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis of rat colon using 60213-1-Ig (same clone as 60213-1-PBS)
rat colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
WB analysis of rat stomach using 60213-1-Ig (same clone as 60213-1-PBS)
rat stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

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Cat No. 60213-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60213-1-PBS targets transgelin/SM22 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

Tested Reactivity	human, rat, pig, mouse, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name	transgelin
Calculated molecular weight	22 kDa
Observed molecular weight	18+22 kDa
GenBank accession number	BC004927
Gene symbol	transgelin/SM22
Gene ID (NCBI)	6876
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS only
Storage Conditions	Store at -80°C.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

transgelin/SM22 Monoclonal antibody, PBS Only

transgelin/SM22 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis of human stomach using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis of pig stomach using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis of rat colon using 60213-1-Ig (same clone as 60213-1-PBS)

WB analysis of rat stomach using 60213-1-Ig (same clone as 60213-1-PBS)

IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

Tested Applications

Recommended dilution

Product Information

Background Information