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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26195-1-AP

Synonyms

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    WB analysis of mouse brain using 26195-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    WB analysis of HeLa using 26195-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    IHC staining of human skin cancer using 26195-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 26195-1-AP (Gamma Tubulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    IF Staining of C2C12 using 26195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    IF Staining of C2C12 using 26195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    IF Staining of MDCK using 26195-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    IF Staining of MDCK using 26195-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Gamma Tubulin Polyclonal antibody (26195-1-AP)

    IF Staining of MDCK using 26195-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26195-1-AP targets Gamma Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Tubulin fusion protein Ag23575 相同性解析による交差性が予測される生物種
Full Name tubulin, gamma 1
Calculated molecular weight 51 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000619
Gene symbol Gamma Tubulin
Gene ID (NCBI) 7283
RRIDAB_2880420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Protocols

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 26195-1-APDownload protocol
IHC protocol for Gamma Tubulin antibody 26195-1-APDownload protocol
IF protocol for Gamma Tubulin antibody 26195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols