uPAR/CD87 Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP00129-3, Human uPAR Recombinant Matched Antibody Pair - PBS only. 83491-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0901. 83491-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

Human peripheral blood mononuclear cells (1 x 10^6 cells/mL) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 5 and assayed for levels of human uPAR.

Serum of sixteen individual healthy human donors was measured. The human uPAR concentration of detected samples was determined to be 3,300.7 pg/mL with a range of 351.4 - 22,799.0 pg/mL.

Cytometric bead array standard curve of MP00129-3, uPAR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83491-1-PBS. Detection antibody: 83491-2-PBS. Standard: Eg0901. Range: 0.313-40 ng/mL.