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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human uPAR/CD87 Rabbit Recombinant Antibody, PBS Only

uPAR/CD87 Recombinant Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

240366G10

Cat no : 98048-1-PBS

Synonyms

PLAUR, UPAR, U PAR, MO3, CD87

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR)

    IF Staining of HeLa using 98048-1-RR (same clone as 98048-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using uPAR/CD87 antibody (98048-1-RR, Clone: 240366G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 98048-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-937 cells using Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR)

    FC experiment of U-937 using 98048-1-RR (same clone as 98048-1-PBS)

    1x10^6 PMA-treated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) (red) or isotype control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) and PE-conjugated Goat Anti-Rabbit IgG (black). Cells were not fixed. This data was developed using the same antibody clone with 98048-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98048-1-RR

    Affinity and Kinetic Characterization of 98048-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98048-1-RR against Human uPAR were performed. The affinity constant is 4.70 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98048-1-PBS targets uPAR/CD87 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name PLAUR
Calculated molecular weight37 kDa
GenBank accession numberNM_002659.4
Gene symbol uPAR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)