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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

uPAR/CD87 Recombinant antibody, PBS Only

uPAR/CD87 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230148A1

Cat no : 82953-1-PBS

Synonyms

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using uPAR/CD87 Recombinant antibody (82953-1-RR)

    IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using uPAR/CD87 Recombinant antibody (82953-1-RR)

    IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-1080 cells using uPAR/CD87 Recombinant antibody (82953-1-RR)

    IF Staining of HT-1080 using 82953-1-RR (same clone as 82953-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using uPAR/CD87 antibody (82953-1-RR, Clone: 230148A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82953-1-RR

    Affinity and Kinetic Characterization of 82953-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82953-1-RR against Human uPAR, PLAUR were performed. The affinity constant is 1.49 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82953-1-PBS targets uPAR/CD87 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen uPAR/CD87 fusion protein Ag28056 相同性解析による交差性が予測される生物種
Full Name PLAUR
Calculated molecular weight 37 kDa
GenBank accession numberBC002788
Gene symbol uPAR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)