PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey). Epifluorescence images were acquired with a 20x objective and post-processed.
PFA-fixed HeLa cells were stained with anti-Actin labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and Sytox Deep Red (cell nuclei, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).