BLI analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Please note the higher apparent affinity owing to avidity effects (dimerization of the cytokine).
ELISA analysis of the capture of varying concentrations of HumanKine IL-6 (HZ-1019) by biotinylated anti-IL-6 VHH (IL6-b). Detection via rabbit polyclonal PTG 21865-1-AP.
BLI analysis of the interaction between varying concentrations of anti-IL-6 VHH and immobilised, biotinylated HumanKine IL-6 (HZ-1019). Fit indicates fitting of data to a 1:1 binding model.