Immunofluorescence with anti-HA-Label, CoraLite ® Plus 488 (hbCL488, 1:500, green) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (blue). Confocal images were were acquired with a 63x oil objective and post-processed.
Multiplex Western Blot with HA-Label (hbCL488, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)
Immunofluorescence with anti-HA-Label, CoraLite ® Plus 555 (hbCL555, 1:500, orange) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (cyan). Confocal images were were acquired with a 63x oil objective and post-processed.
Multiplex Western Blot with HA-Label (hbCL555, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)
Immunofluorescence with anti-HA-Label, CoraLite ® Plus 647 (hbCL647, 1:500, magenta) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (cyan). Confocal images were were acquired with a 63x oil objective and post-processed.
Western Blot with HA-Label (hbCL488, 1:500) targeting HA-labeled TOM70 (black). Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)
The HA-Trap Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution with HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms trimers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.
The HA-Trap Magnetic Agarose was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.
The HA-Trap Magnetic Particles M-270 was used to immunoprecipitate HA-PCNA fusion protein from HEK293T cells. HA-PCNA protein was released from the trap through a two-step competitive elution utizling HA-peptide. Samples from the Input (I), Flow-Through (F), 1st elution (E1), 2nd elution (E2), and residual (R) fractions were analyzed through WB. PCNA Monoclonal Antibody (60097-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001) were used in the WB analysis. Note: PCNA forms timers resulting in co-elution of endogenous PCNA proteins with HA-tagged PCNA.
