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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RICTOR Monoclonal antibody, PBS Only (Capture)

RICTOR Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1C4

Cat no : 66867-2-PBS

Synonyms

Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog, 1D1C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis using 66867-2-Ig (same clone as 66867-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66867-2-Ig (RICTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis of HEK-293 using 66867-2-Ig (same clone as 66867-2-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis of HEK-293 using 66867-2-Ig (same clone as 66867-2-PBS)

    Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis of HeLa using 66867-2-Ig (same clone as 66867-2-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RICTOR Monoclonal antibody (66867-2-Ig)

    IF Staining of NIH/3T3 using 66867-2-Ig (same clone as 66867-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (66867-2-Ig, Clone: 1D1C4 ) at dilution of 1:1700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50234-1

    Cytometric bead array standard curve of MP50234-1

    Cytometric bead array standard curve of MP50234-1, RICTOR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66867-2-PBS. Detection antibody: 66867-3-PBS. Standard:Ag28487. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66867-2-PBS targets RICTOR as part of a matched antibody pair:

MP50234-1: 66867-2-PBS capture and 66867-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RICTOR fusion protein Ag28487 相同性解析による交差性が予測される生物種
Full Name rapamycin-insensitive companion of mTOR
Calculated molecular weight 192 kDa
Observed molecular weight 192 kDa
GenBank accession numberBC029608
Gene symbol RICTOR
Gene ID (NCBI) 253260
RRIDAB_2882204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RICTOR, is a key component of the mTOR complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. RICTOR is the upstream kinase of several AGC kinase family members including AKT, SGK, S6K mutants and several PKC isoforms. Activation of RICTOR-mTORC2 modifies actin organization and promotes cell proliferation and survival. Rictor is overexpressed in several cancers leading to hyperactive mTORC2 and has been shown to play a causal role in glioma formation. Rictor expression has been demonstrated to be regulated transcriptionally and via protein degradation.