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  • Featured Product
  • KD/KO Validated

NDUFB8 Monoclonal antibody, PBS Only (Capture)

NDUFB8 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B12C6

Cat no : 67690-1-PBS

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB8 Monoclonal antibody (67690-1-Ig)

    WB analysis using 67690-1-Ig (same clone as 67690-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using NDUFB8 Monoclonal antibody (67690-1-Ig)

    WB analysis of A549 using 67690-1-Ig (same clone as 67690-1-PBS)

    WB result of NDUFB8 antibody (67690-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells. This data was developed using the same antibody clone with 67690-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NDUFB8 Monoclonal antibody (67690-1-Ig)

    WB analysis using 67690-1-Ig (same clone as 67690-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB8 Monoclonal antibody (67690-1-Ig)

    IHC staining of human liver using 67690-1-Ig (same clone as 67690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB8 Monoclonal antibody (67690-1-Ig)

    IHC staining of human liver using 67690-1-Ig (same clone as 67690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67690-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB8 Monoclonal antibody (67690-1-Ig)

    IHC staining of human kidney using 67690-1-Ig (same clone as 67690-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67690-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50307-1

    Cytometric bead array standard curve of MP50307-1

    Cytometric bead array standard curve of MP50307-1, NDUFB8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67690-1-PBS. Detection antibody: 67690-2-PBS. Standard:Ag6569. Range: 0.098-6.25 ng/mL.

  • Cytometric bead array standard curve of MP50307-2

    Cytometric bead array standard curve of MP50307-2

    Cytometric bead array standard curve of MP50307-2, NDUFB8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67690-1-PBS. Detection antibody: 67690-3-PBS. Standard:Ag6569. Range: 0.098-25 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67690-1-PBS targets NDUFB8 as part of a matched antibody pair:

MP50307-1: 67690-1-PBS capture and 67690-2-PBS detection (validated in Cytometric bead array)

MP50307-2: 67690-1-PBS capture and 67690-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFB8 fusion protein Ag6569 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight18-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
RRIDAB_2882883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.