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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse Il10 Recombinant antibody, PBS Only (Detector)

Il10 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240430G12

Cat no : 82191-3-PBS

Synonyms

IL-10,IL10, IL-10, interleukin 10, Il 10, CSIF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-10 Recombinant antibody (82191-3-RR)

    WB analysis using 82191-3-RR (same clone as 82191-3-PBS)

    Untreated, PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82191-3-RR (Il10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82191-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using IL-10 Recombinant antibody (82191-3-RR)

    IF Staining of RAW 264.7 using 82191-3-RR (same clone as 82191-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Il10 antibody (82191-3-RR, Clone: 240430G12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82191-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00507-2

    Cytometric bead array standard curve of MP00507-2

    Cytometric bead array standard curve of MP00507-2, MOUSE IL-10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82191-4-PBS. Detection antibody: 82191-3-PBS. Standard: Eg0026. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82191-3-PBS targets Il10 as part of a matched antibody pair:

MP00507-2: 82191-4-PBS capture and 82191-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 10
Calculated molecular weight 21 kDa
GenBank accession numberNM_010548
Gene symbol Il10
Gene ID (NCBI) 16153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.