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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD14 Recombinant antibody, PBS Only (Capture)

CD14 Recombinant Antibody for IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240225F2

Cat no : 83297-7-PBS

Synonyms

CD14 antigen, 240225F2

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00313-4

    Sandwich ELISA standard curve of MP00313-4

    Sandwich ELISA standard curve of MP00313-4, Mouse CD14 Monoclonal Matched Antibody Pair - PBS only. 83297-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0847. 83297-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00313-4

    Sample test of MP00313-4

    Mouse splenocytes (1 x 10^6 cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS.The mean CD-14 concentration was determined to be 2,187.4 pg/mL in unstimulated spleen, 8,939.2 pg/mL in IFN-γ and LPS stimulated spleen supernatant.

  • Sample test of MP00313-4

    Sample test of MP00313-4

    Serum of sixteen mice was measured. The CD14 concentration of detected samples was determined to be 113.29 ng/mL with a range of 83.24-160.50 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Recombinant antibody (83297-7-RR)

    IF Staining of RAW 264.7 using 83297-7-RR (same clone as 83297-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-7-RR, Clone: 240225F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83297-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83297-7-RR

    Affinity and Kinetic Characterization of 83297-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83297-7-RR against Mouse CD14 were performed. The affinity constant is 0.838 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83297-7-PBS targets CD14 as part of a matched antibody pair:

MP00313-4: 83297-7-PBS capture and 83297-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD14 antigen
Calculated molecular weight39 kDa
GenBank accession numberNM_009841.4
Gene symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.