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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD4 Recombinant antibody, PBS Only (Capture)

CD4 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240427E10

Cat no : 83513-3-PBS

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E10

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00503-2

    Sandwich ELISA standard curve of MP00503-2

    Sandwich ELISA standard curve of MP00503-2, CD4 Recombinant Matched Antibody Pair - PBS only. 83513-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0333.83513-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00503-2

    Sample test of MP00503-2

    The mean CD4 concentration was determined to be 14.49 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

  • Sample test of MP00503-2

    Sample test of MP00503-2

    Serum of eight individual healthy human donors was measured. The CD4 concentration of detected samples was determined to be 130 pg/mL with a range of 88.46 - 157.66 pg/mL.

  • Cytometric bead array standard curve of MP00503-2

    Cytometric bead array standard curve of MP00503-2

    Cytometric bead array standard curve of MP00503-2, CD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83513-3-PBS. Detection antibody: 83513-4-PBS. Standard: Eg0333. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83513-3-PBS targets CD4 as part of a matched antibody pair:

MP00503-2: 83513-3-PBS capture and 83513-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.