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Host
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bif-1 Recombinant antibody, PBS Only (Capture)

Bif-1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240623E1

Cat no : 83715-1-PBS

Synonyms

SH3GLB1, Endophilin-B1, Endophilin B1, Bif1, Bax-interacting factor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Bif-1 Recombinant antibody (83715-1-RR)

    WB analysis using 83715-1-RR (same clone as 83715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83715-1-RR (Bif-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83715-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Bif-1 Recombinant antibody (83715-1-RR)

    IF Staining of HeLa using 83715-1-RR (same clone as 83715-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bif-1 antibody (83715-1-RR, Clone: 240623E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83715-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00691-2

    Cytometric bead array standard curve of MP00691-2

    Cytometric bead array standard curve of MP00691-2, SH3GLB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83715-1-PBS. Detection antibody: 83715-3-PBS. Standard: Ag7710. Range: 1.25-80 ng/mL.

  • Affinity and Kinetic Characterization of 83715-1-RR

    Affinity and Kinetic Characterization of 83715-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83715-1-RR against Human Bif-1 were performed. The affinity constant is 0.71 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83715-1-PBS targets Bif-1 as part of a matched antibody pair:

MP00691-2: 83715-1-PBS capture and 83715-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bif-1 fusion protein Ag7710 相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like endophilin B1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC007455
Gene symbol Bif-1
Gene ID (NCBI) 51100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).