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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Rat CD90/Thy1 Recombinant antibody, PBS Only (Capture)

CD90/Thy1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241388E2

Cat no : 84206-3-PBS

Synonyms

Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1, CD90

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD90/Thy1 Recombinant antibody (84206-3-RR)

    WB analysis using 84206-3-RR (same clone as 84206-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84206-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CD90/Thy1 Recombinant antibody (84206-3-RR)

    IF Staining of PC-12 using 84206-3-RR (same clone as 84206-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84206-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01132-1

    Cytometric bead array standard curve of MP01132-1

    Cytometric bead array standard curve of MP01132-1, RAT CD90/Thy1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84206-3-PBS. Detection antibody: 84206-1-PBS. Standard: Eg1463. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01132-2

    Cytometric bead array standard curve of MP01132-2

    Cytometric bead array standard curve of MP01132-2, RAT CD90/Thy1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84206-3-PBS. Detection antibody: 84206-2-PBS. Standard: Eg1463. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84206-3-RR

    Affinity and Kinetic Characterization of 84206-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84206-3-PBS targets CD90/Thy1 as part of a matched antibody pair:

MP01132-1: 84206-3-PBS capture and 84206-1-PBS detection (validated in Cytometric bead array)

MP01132-2: 84206-3-PBS capture and 84206-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name Thy-1 cell surface antigen
Calculated molecular weight18 kDa
Observed molecular weight25 kDa
GenBank accession numberNM_012673.2
Gene symbol Thy1
Gene ID (NCBI) 24832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.