Rat IL-4 Recombinant antibody, PBS Only (Capture)

Sandwich ELISA standard curve of MP01293-4, Rat IL-4 Recombinant Matched Antibody Pair - PBS only. 84422-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1113. 84422-5-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

Serum of eight individual healthy human donors was measured. Seven measured less than the lowest rat IL-4 standard, 39.1 pg/mL. One sample read 18.7 pg/mL.

One rat thymus was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of PHA and 50 ng/mL of PMA for 7 days. An aliquot of the cell culture supernatant was removed, assayed for rat IL-4 and measured 98.5 pg/mL.

Cytometric bead array standard curve of MP01293-2, RAT IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84422-3-PBS. Detection antibody: 84422-2-PBS. Standard: Eg1113. Range: 0.625-80 ng/mL.