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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse ALCAM Recombinant antibody, PBS Only (Capture)

ALCAM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241945A4

Cat no : 84528-1-PBS

Synonyms

Protein DM-GRASP, CD166 antigen, CD166, BEN, Activated leukocyte cell adhesion molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using ALCAM Recombinant antibody (84528-1-RR)

    WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALCAM Recombinant antibody (84528-1-RR)

    IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALCAM Recombinant antibody (84528-1-RR)

    IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01379-1

    Cytometric bead array standard curve of MP01379-1

    Cytometric bead array standard curve of MP01379-1, MOUSE ALCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84528-1-PBS. Detection antibody: 84528-3-PBS. Standard: Eg1524. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84528-1-PBS targets ALCAM as part of a matched antibody pair:

MP01379-1: 84528-1-PBS capture and 84528-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight65kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_009655.3
Gene symbol Alcam
Gene ID (NCBI) 11658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).