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PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 14899-1-AP

Synonyms

Multiubiquitin chain-binding protein, MCB1, ASF, AF, 26S proteasome non-ATPase regulatory subunit 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD4 Polyclonal antibody (14899-1-AP)

    WB analysis using 14899-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PSMD4 Polyclonal antibody (14899-1-AP)

    WB analysis of K-562 using 14899-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSMD4 Polyclonal antibody (14899-1-AP)

    WB analysis of HeLa using 14899-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using PSMD4 Polyclonal antibody (14899-1-AP)

    WB analysis of rat spleen using 14899-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMD4 Polyclonal antibody (14899-1-AP)

    IHC staining of human lung cancer using 14899-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMD4 Polyclonal antibody (14899-1-AP)

    IHC staining of human lung cancer using 14899-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMD4 Polyclonal antibody (14899-1-AP)

    IHC staining of human testis using 14899-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMD4 Polyclonal antibody (14899-1-AP)

    IHC staining of human testis using 14899-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSMD4 Polyclonal antibody (14899-1-AP)

    IF Staining of HepG2 using 14899-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMD4 antibody (14899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using PSMD4 Polyclonal antibody (14899-1-AP)

    FC experiment of HepG2 using 14899-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PSMD4 (14899-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inJurkat cells, rat spleen tissue, K-562 cells, HeLa cells, NIH/3T3 cells, RAW264.7 cells, Raji cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14899-1-AP targets PSMD4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag6691 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002365
Gene symbol PSMD4
Gene ID (NCBI) 5710
RRIDAB_2170627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Protocols

Product Specific Protocols
WB protocol for PSMD4 antibody 14899-1-APDownload protocol
IHC protocol for PSMD4 antibody 14899-1-APDownload protocol
IF protocol for PSMD4 antibody 14899-1-APDownload protocol
FC protocol for PSMD4 antibody 14899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Neurosci

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Authors - Rossella Avagliano Trezza
View Article
mouseIF

J Cell Biol

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

Authors - Christoph Gerhardt
View Article
humanWB

Cancer Res

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Authors - Xiuxia Liu
View Article
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A
View Article
humanWB

J Cell Sci

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Authors - Zheng Wang
View Article
humanWB

Mol Cell Proteomics

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Authors - Tatjana Goebel
View Article