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CDK1-Specific Polyclonal antibody

Name: CDK1-Specific Polyclonal antibody
Brand: Proteintech
SKU: 19532-1-AP

CDK1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

205

Review Product

Cat no : 19532-1-AP

Synonyms

CDC2, CDK1, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 19532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19532-1-AP
WB result of CDK1-Specific antibody (19532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1-Specific transfected HeLa cells.
WB analysis using 19532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 19532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 19532-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 19532-1-AP
IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HeLa cells lysate 1600 ug.
IP experiment of HEK-293 using 19532-1-AP
IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HEK-293 cells lysate 1200 ug.
IHC staining of human cervical cancer using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 10x lens).
IHC staining of human cervical cancer using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 40x lens).
IHC staining of rat liver using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 19532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19532-1-AP (CDK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 19532-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1-Specific antibody (19532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 19532-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1-Specific (19532-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 19532-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells
Positive IP detected in	HeLa cells, HEK-293 cells
Positive IHC detected in	human cervical cancer tissue, mouse liver tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 189 publications below
IHC	See 20 publications below
IF	See 8 publications below

Product Information

19532-1-AP targets CDK1-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, pig, chicken, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	cell division cycle 2, G1 to S and G2 to M
Calculated molecular weight	34 kDa
Observed molecular weight	34 kDa
GenBank accession number	NM_001786
Gene symbol	CDK1
Gene ID (NCBI)	983
RRID	AB_10638617
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Protocols

Product Specific Protocols
WB protocol for CDK1-Specific antibody 19532-1-AP	Download protocol
IHC protocol for CDK1-Specific antibody 19532-1-AP	Download protocol
IF protocol for CDK1-Specific antibody 19532-1-AP	Download protocol
IP protocol for CDK1-Specific antibody 19532-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis Authors - Saisai Guo KD Validated View Article
human	IHC	Cell Res Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. Authors - Junya Peng View Article
human	WB	Nat Commun Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells Authors - Qiuhong Zhu View Article
human	WB	Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer Authors - Rong Wang View Article
human	WB	Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. Authors - Jiawei Zhang View Article
human	WB	Nat Commun CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. Authors - Youzhou Sang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK1-Specific Polyclonal antibody

CDK1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 19532-1-AP

WB analysis of HeLa using 19532-1-AP

WB analysis using 19532-1-AP

WB analysis using 19532-1-AP

WB analysis of A431 using 19532-1-AP

IP experiment of HeLa using 19532-1-AP

IP experiment of HEK-293 using 19532-1-AP

IHC staining of human cervical cancer using 19532-1-AP

IHC staining of human cervical cancer using 19532-1-AP

IHC staining of rat liver using 19532-1-AP

IHC staining of mouse liver using 19532-1-AP

IF Staining of HeLa using 19532-1-AP

FC experiment of HeLa using 19532-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma.

Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

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