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  • Featured Product
  • KD/KO Validated

AKT Monoclonal antibody

AKT Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2C5D1

Cat no : 60203-2-Ig

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis using 60203-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of A549 using 60203-2-Ig

    WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells.

  • Western Blot (WB) analysis of HEK-293 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of HEK-293 using 60203-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of rat liver using 60203-2-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of mouse brain using 60203-2-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of HEK-293 using 60203-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of NIH/3T3 using 60203-2-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of RAW 264.7 using 60203-2-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using AKT Monoclonal antibody (60203-2-Ig)

    WB analysis of ROS1728 using 60203-2-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AKT Monoclonal antibody (60203-2-Ig)

    IP experiment of mouse brain using 60203-2-Ig

    IP result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human cervical cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human cervical cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AKT Monoclonal antibody (60203-2-Ig)

    IHC staining of human breast cancer using 60203-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AKT Monoclonal antibody (60203-2-Ig)

    IF Staining of MCF-7 using 60203-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, rat liver tissue, mouse brain tissue, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells, LNCaP cells, Hela cells, Jurkat cells, HSC-T6 cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60203-2-Ig targets AKT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, chicken, bovine, hamster, sheep, duck
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKT fusion protein Ag16695 相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession numberBC000479
Gene symbol AKT1
Gene ID (NCBI) 207
RRIDAB_10912803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 60203-2-IgDownload protocol
IHC protocol for AKT antibody 60203-2-IgDownload protocol
IF protocol for AKT antibody 60203-2-IgDownload protocol
IP protocol for AKT antibody 60203-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang
View Article
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
View Article
mouseWB

Nat Commun

FOXP3+ regulatory T cell perturbation mediated by the IFNγ-STAT1-IFITM3 feedback loop is essential for anti-tumor immunity

Authors - Xinnan Liu
View Article
mouseWB

Cell Rep Med

Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing

Authors - Dongming Lv
View Article
humanWB

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
View Article
mouseWB

Research (Wash D C)

Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis

Authors - Lang Linghu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Janine (Verified Customer) (02-18-2019)

Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: RPE-1 cell lysates (6cm dish)