1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD8a (APC-65069, Clone:53-6.7) or 0.2 ug Isotype Control. Cells were not fixed.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
1x10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 5 ul APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4) or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.
1X10^6 untreated (dashed lines) or LPS-treated (red) mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.
1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1). Cells were not fixed.
1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with FITC Plus Anti-Mouse Ly-6G/Ly-6C (Gr-1) and 0.1 ug APC Anti-Mouse CD11b (APC-65055, Clone: M1/70) or 0.1 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2). Cells were not fixed.
1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD14 and 5 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61), or Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD14 and 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse MHC Class II (I-A/I-E) and 0.2 ug APC Anti-Mouse CD11c (APC-65130, Clone: N418) or 0.2 ug Isotype Control. Cells were not fixed.
Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 10 ul APC Anti-Human CD206 (APC-65155, Clone:15-2) (blue) or APC-Mouse IgG1 isotype control (black). Cells were not fixed.
1X10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were co-surface stained with FITC Plus Anti-Mouse CD4 (GK1.5) and 0.25 ug APC Anti-Mouse CD25 (APC-65137, Clone:PC61.5) or 0.25 ug APC Rat IgG1 Isotype Control (HRPN). Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD4 (APC-65104, Clone: GK1.5) or 0.2 ug Isotype Control. Cells were not fixed.
1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.20 ug APC-Anti-Mouse CD80 (B7-1) (APC-65076, clone 16-10A1) (red) or 0.20 ug APC-Armenian Hamster IgG isotype control (blue). Cells were not fixed.
1X10^6 BALB/c mouse splenocytes were surface stained with PE Anti-Mouse CD4 (GK1.5) and then fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. Cells were then intracellularly stained with 0.06 ug APC Anti-Mouse Foxp3 (APC-65089, Clone:3G3) or 0.06 ug APC Mouse IgG1 Isotype Control (MOPC-21). It is recommended that the antibody dosage should not exceed 0.06ug.
1X10^6 human PBMCs were surface stained with 0.2 ug APC Anti-Mouse IgG2b Isotype Control (APC-65128, Clone:MPC-11) (red), APC Anti-Human CD20 (black), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD44 (APC-65063, Clone: F10-44-2) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human peripheral blood monocytes were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone: 15.2) (blue), or isotype control antibody (black). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD8a (RPA-T8) (APC-65144, Clone:RPA-T8), and 5 ul APC Mouse IgG1 Isotype Control (MOPC-21) (APC-65124, Clone: MOPC-21). Cells were not fixed.
1X10^6 unstimulated or anti-CD3/CD28 treated (2 days) BALB/C mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.2 ug APC Anti-Mouse CD274 (PD-L1, B7-H1) (APC-65073, Clone: 10F.9G2). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD3 (17A2) (APC-65077, Clone:17A2) or 0.1 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2), and 0.1 ug CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5). Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) or 0.1 ug Control Antibody. Cells were not fixed.