HeLa cells were subjected to SDS PAGE followed by western blot with 81760-1-RR (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.
WB result of ATG7 antibody (81760-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells. This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.
IP result of anti-ATG7 (IP:81760-1-RR, 4ug; Detection:81760-1-RR 1:2000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (81760-1-RR, Clone: 2M8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 81760-1-RR against Human ATG7 were performed. The affinity constant is below 1 pM.