Various lysates were subjected to SDS PAGE followed by western blot with 20103-1-AP (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD45 antibody (20103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD45 antibody (20103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP result of anti-CD45 (IP:20103-1-AP, 4ug; Detection:20103-1-AP 1:300) with mouse spleen tissue lysate 4800ug.