Daudi cells were subjected to SDS PAGE followed by western blot with 28331-1-AP (IL4R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28331-1-AP (IL4R antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28331-1-AP (IL4R antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL4R antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
1X10^6 Daudi cells were stained with 0.2 ug CoraLite® Plus 488-conjugated Anti-Human IL-4R (CL488-67051, Clone: 1E4C11) (green), or stained with 0.2 ug isotype control (black). Cells were fixed with 90% MeOH.
1x10^6 Daudi cells were intracellularly stained with 0.8 ug Coralite® Plus 488 IL-4r Polyclonal Antibody (CL488-28331)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL4R antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67051-1-PBS in a different storage buffer formulation.