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85 product results for "CD86"

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CD86 Polyclonal antibody

201 Publications

Reviews (1)

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Various lysates were subjected to SDS PAGE followed by western blot with 13395-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF, ELISA, Cell treatment

Sizes
-_- / -_-

Cat No. 13395-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 13395-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
CD86 Recombinant antibodies (240429G12) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

IHC, WB

Conjugation

Unconjugated

CloneNo.

240429G12
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Cat No. 83523-4-RR, 83523-4-PBS
Anti-Mouse CD86 (GL1) antibodies (10 total products)

30 Publications

Host/IsoType

Rat/IgG2a, kappa

Reactivity

mouse

Applications

FC, IF/ICC

Conjugation

APC, CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, CoraLite®594, FITC Plus, PE, Unconjugated

CloneNo.

GL1
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Cat No. CL488-65068, CL750-65068, APC-65068, CL555-65068, PE-65068, CL647-65068, CL405-65068, FITC-65068, 65068-1-Ig, CL594-65068
CD86 Recombinant antibodies (230476A1) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB

Conjugation

Unconjugated

CloneNo.

230476A1
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Cat No. 83213-5-RR, 83213-5-PBS
Anti-Human CD86 (BU63) Mouse IgG2a Recombinant Antibody

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone: BU63) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Host/IsoType

Mouse/IgG2a

Reactivity

human

Applications

FC

CloneNo.

BU63

Sizes
-_- / -_-

Cat No. 65592-1-MR
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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone: BU63) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone:BU63) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
CD86 (C-terminal) Polyclonal antibody

24 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Sizes
-_- / -_-

Cat No. 26903-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

CD86 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00511-3	83523-5-PBS	83523-6-PBS	CD86	Human	Sandwich ELISA	sELISA: 0.156-10 ng/mL
MP00195-1	83213-3-PBS	83213-4-PBS	Cd86	mouse	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00195-2	83213-2-PBS	83213-3-PBS	Cd86	mouse	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00195-3	83213-2-PBS	83213-1-PBS	Cd86	mouse	Cytometric bead array	CBA: 0.625-20 ng/mL
MP00511-1	83523-3-PBS	83523-1-PBS	CD86	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00511-2	83523-3-PBS	83523-2-PBS	CD86	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP50056-1	68674-1-PBS	68674-2-PBS	CD86	human	Sandwich ELISA	sELISA: 78.1-5000 pg/mL

Anti-Human CD86 (BU63) antibodies (12 total products)

8 Publications

Host/IsoType

Mouse/IgG1, kappa

Reactivity

human, mouse

Applications

FC, IF/ICC

Conjugation

APC, Atlantic Blue™, Cardinal Red™, CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, CoraLite®594, FITC Plus, PE, Unconjugated

CloneNo.

BU63
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Cat No. AB-65165, 65165-1-Ig, CL555-65165, CL647-65165, CL405-65165, APC-65165, CR-65165, CL488-65165, CL750-65165, FITC-65165, PE-65165, CL594-65165
CD86 Polyclonal antibody

4 Publications

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DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse, rat

Applications

WB, IF, ELISA

Sizes
-_- / -_-

Cat No. 30691-1-AP
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DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Anti-Human CD86 (IT2.2) antibodies (6 total products)

Host/IsoType

Mouse/IgG2b, kappa

Reactivity

human

Applications

FC

Conjugation

CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, FITC Plus, Unconjugated

CloneNo.

IT2.2
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Cat No. CL647-65118, CL750-65118, FITC-65118, CL488-65118, 65118-1-Ig, CL555-65118
Anti-Rat CD86 (24F) antibodies (4 total products)

Host/IsoType

Mouse/IgG1, kappa

Reactivity

rat

Applications

FC

Conjugation

CoraLite® Plus 750, CoraLite®594, FITC Plus, Unconjugated

CloneNo.

24F
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Cat No. CL594-65224, 65224-1-Ig, CL750-65224, FITC-65224
CD86 Monoclonal antibodies (1D6H7) (2 total products)

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

1D6H7
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Cat No. 68674-2-Ig, 68674-2-PBS
CD86 Polyclonal antibody

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Daudi cells were subjected to SDS PAGE followed by western blot with 31449-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 31449-1-AP
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Daudi cells were subjected to SDS PAGE followed by western blot with 31449-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Human CD86 ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma

Sensitivity

30.7 pg/mL

Range

78.1-5000 pg/mL

Sizes
-_- / -_-

Cat No. KE00280
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MultiPro® 5CFLX Anti-Human CD86 (BU63)

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G65165-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Host/IsoType

Mouse/IgG1, kappa

Reactivity

human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

CloneNo.

BU63

Sizes
-_- / -_-

Cat No. G65165-1-5C
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G65165-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G65165-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G65165-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

G65165-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.
Recombinant Human B7-2/CD86 protein (His Tag)

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Purity of Recombinant Human B7-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Species

Human

Activity

Immobilized Human B7-2 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 72-290 ng/mL.

Expression

HEK293-derived Human B7-2 protein Leu26-Pro247 (Accession# P42081) with His tag at the C-terminus.

Purity

>95 %, SDS-PAGE

Sizes
-_- / -_-

Cat No. Eg0966
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Purity of Recombinant Human B7-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Human B7-2 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 72-290 ng/mL.
Recombinant Mouse B7-2/CD86 protein (His Tag)

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Purity of Recombinant Mouse B7-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Species

Mouse

Activity

Immobilized Mouse B7-2 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CTLA-4 (hFc tag) with a linear range of 10-40 ng/mL.

Expression

HEK293-derived Mouse B7-2 protein Val24-Glu245 (Accession# P42082-1) with a His tag at the C-terminus.

Purity

>95 %, SDS-PAGE

Sizes
-_- / -_-

Cat No. Eg0802
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Purity of Recombinant Mouse B7-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Mouse B7-2 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CTLA-4 (hFc tag) with a linear range of 10-40 ng/mL.
Anti-Human CD86 (BU63) Recombinant antibodies (4 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC

Conjugation

CoraLite® Plus 647, FITC Plus, Unconjugated

CloneNo.

BU63
-_- / -_-

Cat No. 65514-1-PBS, 65514-1-RR, CL647-65514, FITC-65514
B7-2/CD86 Recombinant antibodies (230476B8) (7 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

FC

Conjugation

CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 647, CoraLite® Plus 750, CoraLite®594, Unconjugated

CloneNo.

230476B8
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Cat No. CL750-98025, CL594-98025, CL647-98025, 98025-1-PBS, 98025-1-RR, CL488-98025, CL405-98025
CD86 Recombinant antibodies (240429C6) (8 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC

Conjugation

CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 647, CoraLite® Plus 750, CoraLite®594, PE, Unconjugated

CloneNo.

240429C6
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Cat No. PE-98043, CL750-98043, CL647-98043, CL488-98043, CL405-98043, 98043-1-RR, 98043-1-PBS, CL594-98043
CD86 Recombinant antibodies (230476B2) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

Cytometric bead array, ELISA, IF/ICC

Conjugation

Unconjugated

CloneNo.

230476B2
-_- / -_-

Cat No. 83213-1-PBS, 83213-1-RR