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27 product results for:

"CD95"

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Fas/CD95 Polyclonal antibody

KO/KD Validated

119 Publications

Reviews (2)

View More Images (3)

Host/IsoType

Rabbit/IgG

Reactivity

hamster, human, monkey, sheep and More (1)

Applications

WB, IP, IF, IHC, CoIP, CHIP, ELISA

Sizes
-_- / -_-

Cat No. 13098-1-AP
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WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells.

Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug.
Fas/CD95 Monoclonal antibody

KO/KD Validated

12 Publications

View More Images (3)

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Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG1

Reactivity

Zebrafish, human

Applications

WB, IF, IHC, ELISA

CloneNo.

1G7F7

Sizes
-_- / -_-

Cat No. 60196-1-Ig
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Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Fas/CD95 Recombinant antibody

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Immunofluorescent analysis of (4% PFA) fixed Raji cells using FAS/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Host/IsoType

Rabbit/IgG

Reactivity

Human

Applications

IF, ELISA

CloneNo.

240340E11

Sizes
-_- / -_-

Cat No. 83400-5-RR
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Immunofluorescent analysis of (4% PFA) fixed Raji cells using FAS/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Immunofluorescent analysis of (4% PFA) fixed Raji cells using FAS/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Biolayer interferometry (BLl) kinetic assays of 83400-5-RR against Human Fas/CD95 were performed. The affinity constant is 0.927 nM.
Recombinant Human Fas/CD95protein (His Tag)

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Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Species

Human

Activity

Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.

Expression

HEK293-derived Human Fas protein Gln26-Asn173 (Accession# P25445-1) with His tag at the C-terminus.

Purity

>95 %, SDS-PAGE

Sizes
-_- / -_-

Cat No. Eg0877
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Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.
Anti-Human Fas/CD95 Rabbit Recombinant Antibody

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed.

Host/IsoType

Rabbit/IgG

Reactivity

Human

Applications

FC

CloneNo.

240340E11

Sizes
-_- / -_-

Cat No. 98034-1-RR
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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed.

1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) (red), or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Anti-Human Fas/CD95 Rabbit Recombinant Antibody

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed.

Host/IsoType

Rabbit/IgG

Reactivity

Human

Applications

FC

CloneNo.

240340G11

Sizes
-_- / -_-

Cat No. 98034-2-RR
×

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed.

1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11)(red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
CoraLite® Plus 488-conjugated Fas/CD95 Polyclonal antibody

KO/KD Validated

Enlarge Image

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1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FAS/CD95 (CL488-13098) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488

Sizes
-_- / -_-

Cat No. CL488-13098
×

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1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FAS/CD95 (CL488-13098) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Fas/CD95 Monoclonal antibody, PBS Only

KO/KD Validated

View More Images (3)

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Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

WB, IHC, ELISA

CloneNo.

1G7F7

Sizes
-_- / -_-

Cat No. 60196-1-PBS
×

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More validation images available on the product page

Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.
Fas/CD95 Monoclonal antibody, PBS Only (Capture)

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Cytometric bead array standard curve of MP50205-1, Fas/CD95 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68828-1-PBS. Detection antibody: 68828-2-PBS. Standard:Eg0877. Range: 0.781-100 ng/mL.

Host/IsoType

Mouse/IgG1

Reactivity

Human

Applications

Indirect ELISA, Cytometric bead array

CloneNo.

3F1A2

Sizes
-_- / -_-

Cat No. 68828-1-PBS
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Cytometric bead array standard curve of MP50205-1, Fas/CD95 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68828-1-PBS. Detection antibody: 68828-2-PBS. Standard:Eg0877. Range: 0.781-100 ng/mL.

Cytometric bead array standard curve of MP50205-2, Fas/CD95 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68828-1-PBS. Detection antibody: 68828-3-PBS. Standard:Eg0877. Range: 1.563-100 ng/mL.

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抗体

免疫アッセイ

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Species Reactivity

Species Reactivity

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Conjugate

27 product results for:

"CD95"

Fas/CD95 Polyclonal antibody

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Fas/CD95 Monoclonal antibody

-_- / -_-

Fas/CD95 Recombinant antibody

-_- / -_-

Recombinant Human Fas/CD95protein (His Tag)

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Anti-Human Fas/CD95 Rabbit Recombinant Antibody

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Anti-Human Fas/CD95 Rabbit Recombinant Antibody

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CoraLite® Plus 488-conjugated Fas/CD95 Polyclonal antibody

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Fas/CD95 Monoclonal antibody, PBS Only

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Fas/CD95 Monoclonal antibody, PBS Only (Capture)

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