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90 product results for "CTLA-4"

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CTLA-4/CD152 Polyclonal antibody

1 Publications

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Untreated or Con A-treated (3 ug/ml, 72h) mouse splenocytes were subjected to SDS PAGE followed by western blot with 30648-1-AP (CTLA-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

WB, IF-P, ELISA

Sizes
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Cat No. 30648-1-AP
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Untreated or Con A-treated (3 ug/ml, 72h) mouse splenocytes were subjected to SDS PAGE followed by western blot with 30648-1-AP (CTLA-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CTLA-4 antibody (30648-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1, red).
CTLA4 VHHs (4 total products)

Applications

FC, IF/ICC

Conjugation

CoraLite® Plus 555, CoraLite® Plus 647, FITC Plus, Unconjugated
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Cat No. FITC-ctl, CL647-ctl, CL555-ctl, ctlCys2

CTLA-4/CD152 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00736-4	83778-6-PBS	83778-3-PBS	CTLA-4/CD152	Human	Sandwich ELISA	sELISA: 23.4-1500 pg/mL
MP00736-1	83778-1-PBS	83778-5-PBS	CTLA-4/CD152	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00736-2	83778-4-PBS	83778-5-PBS	CTLA-4/CD152	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00736-3	83778-2-PBS	83778-3-PBS	CTLA-4/CD152	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP50106-1	68751-3-PBS	68751-4-PBS	CTLA-4/CD152	human	Cytometric bead array	CBA: 3.125-100 ng/mL
MP50106-2	68751-1-PBS	68751-2-PBS	CTLA-4/CD152	human	Cytometric bead array	CBA: 0.391-50 ng/mL

Recombinant Human CTLA-4 protein (hFc Tag)

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Purity of Recombinant Human CTLA-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Species

Human

Activity

Immobilized Human CD86 (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human CTLA-4 (hFc tag) with a linear range of 5-22 ng/mL.

Expression

HEK293-derived Human CTLA-4 protein Lys36-Asp161 (Accession# P16410) with a human IgG1 Fc tag at the C-terminus.

Purity

>95 %, SDS-PAGE

Sizes
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Cat No. Eg1004
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Purity of Recombinant Human CTLA-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Human CD86 (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human CTLA-4 (hFc tag) with a linear range of 5-22 ng/mL.
CTLA-4/CD152 Recombinant antibodies (240793E8) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC

Conjugation

Unconjugated

CloneNo.

240793E8
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Cat No. 98078-1-PBS, 98078-1-RR
CTLA4 E.Coli expressed Tagged-proteins (2 total products)

Species

human

Source

PGEX-4T

Tag

GST
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Cat No. Ag28226, Ag24178
CD86 Polyclonal antibody

190 Publications

Reviews (1)

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Various lysates were subjected to SDS PAGE followed by western blot with 13395-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF, ELISA, Cell treatment

Sizes
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Cat No. 13395-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 13395-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
CD86 Recombinant antibodies (240429G12) (2 total products)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

IHC, WB

Conjugation

Unconjugated

CloneNo.

240429G12
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Cat No. 83523-4-PBS, 83523-4-RR
CD80/B7-1 Monoclonal antibodies (1E2F10) (2 total products)

52 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC, WB

Conjugation

Unconjugated

CloneNo.

1E2F10
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Cat No. 66406-1-PBS, 66406-1-Ig
Anti-Human CD86 (BU63) Mouse IgG2a Recombinant Antibody

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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone: BU63) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Host/IsoType

Mouse/IgG2a

Reactivity

human

Applications

FC

CloneNo.

BU63

Sizes
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Cat No. 65592-1-MR
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1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone: BU63) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Mouse IgG2a Recombinant Antibody (65592-1-MR, Clone:BU63) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
CD80/B7-1 Polyclonal antibody

30 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, ELISA

Sizes
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Cat No. 14292-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
CD86 (C-terminal) Polyclonal antibody

24 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Sizes
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Cat No. 26903-1-AP
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Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

CD86 Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00511-3	83523-5-PBS	83523-6-PBS	CD86	Human	Sandwich ELISA	sELISA: 0.156-10 ng/mL
MP00511-1	83523-3-PBS	83523-1-PBS	CD86	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00511-2	83523-3-PBS	83523-2-PBS	CD86	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP50056-1	68674-1-PBS	68674-2-PBS	CD86	human	Sandwich ELISA	sELISA: 78.1-5000 pg/mL

CD80 Matched Antibody Pairs^New

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00284-1	83198-2-PBS	83198-1-PBS	CD80	HUMAN	Cytometric bead array	CBA: 0.625-20 ng/mL
MP50098-1	68746-1-PBS	68746-2-PBS	CD80	HUMAN	Cytometric bead array, Sandwich ELISA	CBA: 0.391-100 ng/mL

Anti-Human CD86 (BU63) antibodies (12 total products)

6 Publications

Host/IsoType

Mouse/IgG1, kappa

Reactivity

human, mouse

Applications

FC, IF/ICC

Conjugation

APC, Atlantic Blue™, Cardinal Red™, CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, CoraLite®594, FITC Plus, PE, Unconjugated

CloneNo.

BU63
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Cat No. CL405-65165, CL750-65165, 65165-1-Ig, CR-65165, PE-65165, CL488-65165, CL594-65165, AB-65165, FITC-65165, APC-65165, CL555-65165, CL647-65165
Anti-Human CD80 (B7-1) antibodies (3 total products)

3 Publications

Host/IsoType

Mouse/IgG1, kappa

Reactivity

human

Applications

FC

Conjugation

CoraLite® Plus 647, PE, Unconjugated

CloneNo.

2D10.4
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Cat No. PE-65083, CL647-65083, 65083-1-Ig
Anti-Human CD86 (IT2.2) antibodies (6 total products)

Host/IsoType

Mouse/IgG2b, kappa

Reactivity

human

Applications

FC

Conjugation

CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, FITC Plus, Unconjugated

CloneNo.

IT2.2
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Cat No. CL555-65118, 65118-1-Ig, FITC-65118, CL647-65118, CL488-65118, CL750-65118
CD86 Monoclonal antibodies (1D6H7) (2 total products)

Host/IsoType

Mouse/IgG1

Reactivity

human

Applications

ELISA, WB

Conjugation

Unconjugated

CloneNo.

1D6H7
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Cat No. 68674-2-Ig, 68674-2-PBS
CD86 Polyclonal antibody

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Daudi cells were subjected to SDS PAGE followed by western blot with 31449-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 31449-1-AP
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Daudi cells were subjected to SDS PAGE followed by western blot with 31449-1-AP (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Human CD86 ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma

Sensitivity

30.7 pg/mL

Range

78.1-5000 pg/mL

Sizes
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Cat No. KE00280
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MultiPro® 5CFLX Anti-Human CD86 (BU63)

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G65165-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Host/IsoType

Mouse/IgG1, kappa

Reactivity

human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

CloneNo.

BU63

Sizes
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Cat No. G65165-1-5C
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G65165-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G65165-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G65165-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

G65165-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTLA-4/CD152 Polyclonal antibody

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CTLA4 VHHs (4 total products)

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CTLA-4/CD152 Matched Antibody PairsNew

Recombinant Human CTLA-4 protein (hFc Tag)

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CTLA-4/CD152 Recombinant antibodies (240793E8) (2 total products)

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CTLA4 E.Coli expressed Tagged-proteins (2 total products)

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CD86 Polyclonal antibody

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CD86 Recombinant antibodies (240429G12) (2 total products)

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CD80/B7-1 Monoclonal antibodies (1E2F10) (2 total products)

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Anti-Human CD86 (BU63) Mouse IgG2a Recombinant Antibody

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CD80/B7-1 Polyclonal antibody

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CD86 (C-terminal) Polyclonal antibody

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CD86 Matched Antibody PairsNew

CD80 Matched Antibody PairsNew

Anti-Human CD86 (BU63) antibodies (12 total products)

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Anti-Human CD80 (B7-1) antibodies (3 total products)

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Anti-Human CD86 (IT2.2) antibodies (6 total products)

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CD86 Monoclonal antibodies (1D6H7) (2 total products)

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CD86 Polyclonal antibody

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Human CD86 ELISA Kit

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MultiPro® 5CFLX Anti-Human CD86 (BU63)

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Products derived from

CTLA-4/CD152 Matched Antibody Pairs^New

CD86 Matched Antibody Pairs^New

CD80 Matched Antibody Pairs^New