Various lysates were subjected to SDS PAGE followed by western blot with 10434-1-AP (DIABLO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB result of DIABLO antibody (10434-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIABLO transfected HeLa cells.
IP result of anti-DIABLO (IP:10434-1-AP, 4ug; Detection:10434-1-AP 1:2000) with HepG2 cells lysate 880 ug.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10434-1-AP (DIABLO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIABLO antibody (10434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).