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Fusion Proteins

Host

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Chicken
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Species Reactivity

Human
Mouse
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Conjugate

APC
Biotin
CoraLite®405
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CoraLite®555
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AP
Biotin
CoraLite®488
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Compatible Species

Rabbit
Mouse

Conjugate

CoraLite Plus 405
FITC Plus^NEW
CoraLite Plus 488
CoraLite Plus 555
CoraLite Plus 647
CoraLite Plus 750

Target

Host

Alpaca
Donkey
Goat
Mouse
Rabbit

Species Reactivity

Chicken
Goat
Guinea Pig
Horse
Human
Mouse
Rabbit
Rat
Sheep
Swine

Species Reactivity

Human
Mouse
Rat
Pig
Bovine
Chicken
Zebrafish
Hamster
Monkey
Drosophila

Conjugate

APC
Biotin
CoraLite®405
CoraLite®488
CoraLite®532
CoraLite®555
CoraLite®568
CoraLite®594
CoraLite®647
CoraLite®650
FITC
HRP
PE

Conjugate

Alexa Fluor® 488
Alexa Fluor® 568
Alexa Fluor® 647
AP
Biotin
CoraLite®488
CoraLite®594
CoraLite®647
Cy3
FITC
HRP
Rhodamine
R-PE

20 product results for "DYKDDDDK"

Filters

Clear All

Target

DYKDDDDK (2)
DYKDDDDK tag (14)

Applications

IF/ICC (12)
WB (10)
IP (7)
CoIP (5)
RIP (4)
ChIP (3)
IHC (3)
ELISA (2)
FC (Intra) (2)

Conjugation

Unconjugated (7)
Agarose (3)
CoraLite® Plus 647 (2)
CoraLite® Plus 750 (2)
Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) (1)
Biotin (1)
CoraLite® Plus 488 (1)
CoraLite®594 (1)
FITC (1)
HRP (1)

Clonality

Monoclonal (10)
Recombinant (4)
Polyclonal (2)

CloneNo.

8H6A10 (7)
2B3C4 (2)
4K14 (2)
1E7B4 (1)
240568A11 (1)

Reactive Species

recombinant protein (14)
human (6)
mouse (4)
duck (3)
pig (3)
canine (2)
chicken (2)
monkey (2)
rat (2)
zebrafish (2)
hamster (1)
sheep (1)
yeast (1)

Host Species

Mouse (11)
Rabbit (5)
E.coli (1)

Buffer formulation

PBS and Azide (13)
PBS Only (2)

Product Types

Primary Antibodies (15)

Hybridoma derived-monoclonals (10)
Polyclonal (2)
Recombinant (3)

Recombinant Proteins (1)

E.Coli expressed Tagged-proteins (1)

Kits & Assays (4)

Nano-Traps and Nanobody-based Kits (3)
Supporting Reagents (1)

Filters

CloneNo.

1E7B4 (1)
240568A11 (1)
2B3C4 (2)
4K14 (2)
8H6A10 (7)

Product Types

E.Coli expressed Tagged-proteins (1)
Hybridoma derived-monoclonals (10)
Nano-Traps and Nanobody-based Kits (3)
Polyclonal (2)
Recombinant (3)
Supporting Reagents (1)

Conjugation

Agarose (3)
Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) (1)
Biotin (1)
CoraLite® Plus 488 (1)
CoraLite® Plus 647 (2)
CoraLite® Plus 750 (2)
CoraLite®594 (1)
FITC (1)
HRP (1)
Unconjugated (7)

Reactive Species

canine (2)
chicken (2)
duck (3)
hamster (1)
human (6)
monkey (2)
mouse (4)
pig (3)
rat (2)
recombinant protein (14)
sheep (1)
yeast (1)
zebrafish (2)

Applications

ChIP (3)
CoIP (5)
ELISA (2)
FC (Intra) (2)
IF/ICC (12)
IHC (3)
IP (7)
RIP (4)
WB (10)

Target

DYKDDDDK (2)
DYKDDDDK tag (14)

Host Species

E.coli (1)
Mouse (11)
Rabbit (5)

Clonality

Monoclonal (10)
Polyclonal (2)
Recombinant (4)

Buffer formulation

PBS and Azide (13)
PBS Only (2)

DYKDDDDK tag Monoclonal antibodies (8H6A10) (7 total products)

312 Publications

Reviews (3)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, canine, chicken, duck, hamster, monkey, pig, recombinant protein, zebrafish

Applications

ChIP, CoIP, ELISA, FC (Intra), IF/ICC, IHC, IP, RIP, WB

Conjugation

Biotin, CoraLite® Plus 647, CoraLite®594, FITC, HRP, Unconjugated

CloneNo.

8H6A10
-_- / -_-

Cat No. HRP-66008, CL594-66008, 66008-4-PBS, FITC-66008, Biotin-66008-4, 66008-4-Ig, CL647-66008
CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

Enlarge Image

×
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.

Host/IsoType

Mouse/IgG2b

Reactivity

recombinant protein

Applications

IF/ICC

Conjugate

CoraLite® Plus 488

CloneNo.

1E7B4

Sizes
-_- / -_-

Cat No. CL488-66008
×

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More validation images available on the product page

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.
DYKDDDDK tag Polyclonal antibodies (2 total products)

1010 Publications

Reviews (20)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken, duck, monkey, pig, recombinant protein, sheep, yeast, zebrafish

Applications

ChIP, CoIP, IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 750, Unconjugated
-_- / -_-

Cat No. CL750-20543, 20543-1-AP
DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

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×
TDP-43-flag Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80801-2-RR (Ag2329 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

CloneNo.

240568A11

Sizes
-_- / -_-

Cat No. 80801-2-RR
×

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More validation images available on the product page

TDP-43-flag Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80801-2-RR (Ag2329 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IP result of anti-DYKDDDDK tag (IP:80801-2-RR, 4ug; Detection:80801-2-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug.

1x10^6 HEK-293T transfected with TDP-43-flag were intracellularly stained with 0.4 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80801-2-RR, Clone:240568A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293T cells using DYKDDDDK tag antibody (80801-2-RR, Clone: 240568A11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Biolayer interferometry (BLl) kinetic assays of 80801-2-RR against DYKDDDDK tag were performed. The affinity constant is 15.3 pM.
DYKDDDDK-Fab-Traps (3 total products)

33 Publications

Reviews (7)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
-_- / -_-

Cat No. ffap, ffak, ffa
3xDYKDDDDK-peptide

1 Publications

Enlarge Image

×
DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

Application

Elution

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. fp
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.
DYKDDDDK tag Recombinant antibodies (4K14) (2 total products)

1 Publications

Host/IsoType

Rabbit/IgG

Reactivity

human, recombinant protein

Applications

IF/ICC, WB

Conjugation

CoraLite® Plus 647, CoraLite® Plus 750

CloneNo.

4K14
-_- / -_-

Cat No. CL750-80010, CL647-80010
DYKDDDDK tag Fusion Protein (Contains same sequence as FLAG® Tag)

Enlarge Image

Source

E. coli-derived, PGEX-4T

Tag

GST

Format

Powder

Sizes
-_- / -_-

Cat No. Ag2329
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

DYKDDDDK tag fusion protein Ag2329 SDS-PAGE
DYKDDDDK tag Monoclonal antibodies (2B3C4) (2 total products)

199 Publications

Reviews (7)

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, canine, duck, pig, recombinant protein

Applications

CoIP, ELISA, IF/ICC, IHC, IP, RIP, WB

Conjugation

Unconjugated

CloneNo.

2B3C4
-_- / -_-

Cat No. 66008-3-Ig, 66008-3-PBS